Project Name: Magnetic-luminescent Fe-Si hybrid nanoparticles designed for biomedical aplications (EN)

Contract no. 114/2016

Project code: PN-III-P2-2.1-PED-2016-1698

Program: Pr. 2 – Competitiveness increasing of Romanian economy through research, development and innovation.

Project type: Experimental-demonstrative project

Project director: Dumitrache Florian

Coordinator: National Institute for Lasers, Plasma, and Radiation Physics

Financing Authority: Executive Unit for Financing Higher Education, Research, Development and Innovation (UEFISCDI)

Project summary:

The project intends to develop and optimize magnetically-operable, luminescent nanoparticles for multifunctional purposes (diagnosis, drug distribution, hyperthermia and photodynamic treatment) based on hybrid Fe-Si nanoparticles. The development of the original laboratory-validated system consists on obtaining a stabilized and bio-tested suspension based on Fe-Si nanoparticles synthesized by laser pyrolysis, in a single step. The project proposes two nanostructure configurations: aggregated nanoparticles containing separate Si and Fe nanocrystals or Fe-core composite nanoparticles covered in Si-based shell. Thus, a dual material is obtained: magnetic (virtue of Fe) and luminescent (Q-dot Si). It is intended to adapt the average size of Si nanocrystals to values between 2-5 nm in order to generate visible luminescence. The nanoparticles produced will be dispersed and stabilized using derivatives of PEG, L-Dopa, CMCNa, threonine, dextran, chitosan, etc. Finally, the optimized product demonstration model will be validated on the basis of 6 characteristics: nanoparticle size, magnetic response parameters, photoluminescence efficiency, suspension stability, biocompatibility, and magnetic induced hyperthermia effect.

Phase I - 29/12/2017
Iron based and hybrid Fe / Si nanocrystals synthesized by laser pyrolysis: structure, magnetic and biocompatibility analysis
Please click here to view phase details

Phase I Duration: 03/01/2017 - 29/12/2017
Value allocated to Phase I from the state budget for 2017: 406,415 RON.


The results obtained during this phase were presented at 2
International Conferences, and were brought together in 2 Scientific

• L. Gavrila-Florescu, F. Dumitrache, M.Balas, C.T. Fleaca, M.Scarisoreanu, I.P. Morjan, E. Dutu, A. Ilie, A.-M. Banici, C. Locovei, G. Prodan, "Synthesis of Fe-based Core @ ZnO shell nanopowders by laser pyrolysis for biomedical applications", Applied Physics A (2017) 123: 802.
• A review article "Synthesis, characterization and in vitro biological evaluation of hybrid Fe / Si nanoparticles" at the Journal of Nanoparticle Research (Nano-D-17-02078).

International Conferences:
• Poster presentation: L. Gavrila-Florescu, F. Dumitrache, M. Balas, C. Fleaca, C. Locovei, I.P. Morjan, M.Scarisoreanu, A. Ilie, A. Banici, G. Prodan, "Synthesis by laser pyrolysis of Fe based core @ ZnO shell nanopowders for biomedical applications", EMRS Spring Meeting 2017, June 21-27, France - Strasbourg, Symposium X.
• Oral presentation: F. Dumitrache, M. Balas, C. Fleaca, I. Morjan, A. Dimischiotu, M.S. Stan, I. Sandu, A. Ilie, C. Locovei, IP Morjan, E. Vasile, O. Marinica, "Magnetic and biocompatible tests of novel iron nitride based nanoparticles synthesized by ammonia sensitized laser pyrolysis", EMRS Spring Meeting 2017, 21 June 27, France - Strasbourg,
Symposium X.


Florian Dumitrache
Claudiu Fleaca
Ion Morjan
Elena Dutu
Alina Ilie
Ion Sandu
Iuliana Morjan
Eugenia Vasile
Adrian Petris
Mihai Patachia
Dan Adrian Miron
Stefan George Banita

Anca Dinischiotu
Sorina Nicoleta Voicu
Miruna Silvia Stan
Virginia Cecilia Gheran
Mihaela Balas
Claudia Ion

Phase II– 30/06/2018
Iron based and hybrid Fe / Si nanocrystals synthesized by laser pyrolysis: structure, magnetic and biocompatibility analysis

Phase II Duration: 1/01/2018 — 30/06/2018

Value allocated to Phase II from the state budget for 2018: 193,585 RON.

During Phase 2 a series of 4 activities were carried out aiming at optimizing the laser pyrolysis technique for magnetic and luminescent Fe / Si based hybrid nanomaterials production. The nanoparticles were tested with stabilizers and we optimized stabilized suspension preparation recipes which in the end were adapted to an identical base fluid in PBS or FBS composition for subsequent cytotoxicity testing, relative to cellular penetration capacity. We also used the stabilized suspensions of hybrid nanopowders to prepare opaque inverse photonic devices and polyacrylic gels. We report the successful synthesis of aggregates with distinct Fe and Si (Fe-Si) hybrid nanoparticles, through an unused original method. Synthesized nanopowders under optimal conditions show high magnetisations (up to 65 emu / g) and luminescence in the visible-red or near IR spectra, even at a visible excitation (green-530 nm). Both synthesized powders and solid part of the stabilized suspensions are morpho-structurally analysed by XRD, TEM, SAED, EDS, Raman, while the stabilization quality is assessed with DLS and TEM. Magnetic properties, luminescence and hyperthermia are evaluated. Stabilized suspensions under optimal conditions exhibited a very good dispersion in culture medium, especially those containing tumour cells, which facilitated good cellular penetration even after 24 hours of exposure. In addition, cell morphology and viability did not change significantly, indicating a high degree of biocompatibility for the analysed nanostructures. Biochemical assessments clearly indicate an oxidative starch in tumour cells, a premise for future anticancer therapy based on these Fe-Si hybrid nanoparticles.


1. Laser pyrolysis synthesis of Fe-Si hybrid nanopowders with optimized structural and biocompatibility.
2. Hybrid Fe-Si nanoparticles stabilized dispersions preparation (with PBS, FBS, etc.) prepared to be added to cell cultures: DLS measurements of the mean hydrodynamic diameter evolution over time.
3. In vitro study of biocompatibility, cellular penetration, oxidative stress and induced magnetic hyperthermia on stabilized dispersions of optimized nanoparticles in magnetic and photo-immune parameters
4. Dissemination


The results obtained during this phase were presented at 2 International Conferences, and were brought together in 3 Scientific Articles:

• Mihaela Balas, Florian Dumitrache, Madalina A. Badea, Claudiu Fleaca, Anca Badoi, Eugenia Vasile, Anca Dinischiotu. Coating dependent in vitro biocompatibility of new Fe-Si nanoparticles. Nanomaterials 2018, in revison.
• Ion Sandu, Marius Dumitru, Claudiu Teodor Fleaca, Florian Dumitrache Rise and side infiltration in opals and porous materials for their skin-free replica synthesis Photonics and Nanostructures, Fundamentals and Applications 2018 submited.
• D. Zdrentan, Calu Larisa, Mihali Ciprian Valentin, Tusa Iris Maria, Florian Dumitrache, Cotoraci Coralia Adina, Daniela Bratosin „Characterisation of human erytrocites as carriers for iron nanoparticles” Romanian Biotechnological Letters 2018 submited.

International Conferences:

The 10th Congress of Toxicology in Developing Countries and 12th Serbian Congress of Toxicology, Belgrad, Serbia, 18-21 aprilie 2018:
• Biological responses to hybrid Fe-Si nanoparticles in Caco2 cells. Book of Abstracts, p. 136. Mihaela Balas, Florian Dumitrache, Madalina Andreea Badea, Andreea Luminita Radulescu, Claudiu Fleaca, Claudiu Locovei, Eugenia Vasile, Anca Dinischiotu

EMRS Spring Meeting Strasbourg Franta 18-22 iunie 2018 Simpozionul X “Photon-assisted synthesis and processing of materials in micro-nanoscale”:

• Cod X PII.12 “Soft nanocomposites: Hydrogel matrix containing iron based nanoparticles synthesized by laser pyrolysis : magnetic and hyperthermic properties”, autori C. Fleaca, F. Dumitrache, C. Locovei, A.-M. Banici, A. Badoi, A. Ilie, S. Banita, E. Vasile, L. Vekas
• Cod X PII26 “Magnetic, luminescent and biocompatible tests on as synthesized or thermal treated Fe-Si hybrid nanoparticles prepared by laser pyrolysis”, autori F. Dumitrache, C. Fleaca, M. Balas, I. Morjan, A. Ilie, A. Dinischiotu, E. Tanasa, M. Enculescu, C. Locovei, C. Mihailescu, O. Marinica